Automated Cell-Free Multiprotein Synthesis Facilitates the Identification of a Secretory, Oligopeptide Elicitor-Like, Immunoreactive Protein of the Oomycete Pythium insidiosum.

Automated Cell-Free Multiprotein Synthesis Facilitates the Identification of a Secretory, Oligopeptide Elicitor-Like, Immunoreactive Protein of the Oomycete Pythium insidiosum.

Protein manufacturing depends on time-consuming genetic engineering and in vivo expression, which is a bottleneck for purposeful research within the postgenomic period. Cell-free protein synthesis (CFPS) overcomes the limitation of in vivo protein biosynthesis by processing in vitro transcription and translation of a number of genes to proteins inside hours. We employed an automatic CFPS to concurrently synthesize proteins from 24 genes of […]

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