Automated Cell-Free Multiprotein Synthesis Facilitates the Identification of a Secretory, Oligopeptide Elicitor-Like, Immunoreactive Protein of the Oomycete Pythium insidiosum.

Automated Cell-Free Multiprotein Synthesis Facilitates the Identification of a Secretory, Oligopeptide Elicitor-Like, Immunoreactive Protein of the Oomycete Pythium insidiosum.

Protein manufacturing depends on time-consuming genetic engineering and in vivo expression, which is a bottleneck for purposeful research within the postgenomic period. Cell-free protein synthesis (CFPS) overcomes the limitation of in vivo protein biosynthesis by processing in vitro transcription and translation of a number of genes to proteins inside hours. We employed an automatic CFPS to concurrently synthesize proteins from 24 genes of the oomycete Pythium insidiosum (which causes the life-threatening illness pythiosis) and display screen for a diagnostic and therapeutic goal. CFPS efficiently synthesized 18 proteins (∼75% success price).

One protein, particularly, I06, was explicitly acknowledged by all pythiosis sera, however not management sera, examined. Py. insidiosum secreted a big quantity of I06. The protein structure of I06 is appropriate with the oligopeptide elicitor (OPEL) of the phylogenetically associated plant-pathogenic oomycete Phytophthora parasitica The OPEL-like I06 protein of Py. insidiosum can stimulate host antibody responses, much like the P. parasitica OPEL that triggers plant protection mechanisms. OPEL-like I06 homologs are current solely within the oomycetes.

Py. insidiosum accommodates two OPEL-like I06 homologs, however solely one of many two homologs was expressed throughout hyphal development. Twenty-nine homologs derived from 15 oomycetes could be phylogenetically divided into two teams. The OPEL-like genes would possibly happen within the widespread ancestor, earlier than independently present process gene achieve and loss throughout the oomycete speciation. In conclusion, CFPS presents a quick in vitro protein synthesis. CFPS concurrently generated a number of proteins of Py. insidiosum and facilitated the identification of the secretory OPEL-like I06 protein, a possible goal for the event of a management measure towards the pathogen.

Technical limitations of standard biotechnological strategies (i.e., genetic engineering and protein synthesis) forestall in depth purposeful research of the large quantities of genetic info out there in the present day. We employed a cell-free protein synthesis system to quickly and concurrently generate a number of proteins from genetic codes of the oomycete Pythium insidiosum, which causes the life-threatening illness referred to as pythiosis, in people and animals worldwide. We aimed to display screen for potential diagnostic and therapeutic protein targets of this pathogen. Eighteen proteins have been synthesized.

Of the 18 proteins, one was a secreted immunoreactive protein, referred to as I06, that triggered host immunity and was acknowledged explicitly by all examined sera from pythiosis sufferers. It is among the OPEL proteins; these proteins are current solely within the distinctive group of microorganisms referred to as oomycetes. Right here, we demonstrated that cell-free protein synthesis was helpful for the manufacturing of a number of proteins to facilitate purposeful research and establish a possible goal for prognosis and therapy of pythiosis.

A brand new neuropeptide insect parathyroid hormone iPTH within the crimson flour beetle Tribolium castaneum.

Within the postgenomics period, comparative genomics have superior the understanding of evolutionary processes of neuropeptidergic signaling techniques. The evolutionary origin of many neuropeptidergic signaling techniques could be traced date again to early metazoan evolution based mostly on the conserved sequences. Insect parathyroid hormone receptor (iPTHR) was beforehand described as an ortholog of vertebrate PTHR that has a well known operate in controlling bone reworking. Nonetheless, there was no sequence homologous to PTH sequence in insect genomes, leaving the iPTHR as an orphan receptor.

Right here, we recognized the genuine ligand insect PTH (iPTH) for the iPTHR. The taxonomic distribution of iPTHR, which is missing in Diptera and Lepidoptera, supplied a lead for figuring out the genuine ligand. We discovered {that a} beforehand described orphan ligand referred to as PXXXamide (the place X is any amino acid) described within the cuttlefish Sepia officinalis has an identical taxonomic distribution sample as iPTHR. Checks of this peptide, iPTH, in purposeful reporter assays confirmed the interplay of the ligand-receptor pair. Research of a mannequin beetle

Tribolium castaneum, was used to analyze the operate of the iPTH signaling system by RNA interference adopted by RNA sequencing and phenotyping. The outcomes urged that the iPTH system is probably going concerned within the regulation of cuticle formation that culminates with a phenotype of defects in wing exoskeleton maturation on the time of grownup eclosion. Furthermore, RNAi of iPTHRs additionally led to vital reductions in egg numbers and hatching charges after parental RNAi. Regardless of herculean efforts using a variety of antibiotic discovery platform methods, together with genomics, bioinformatics, techniques biology and postgenomic approaches, success has been at greatest incremental.

Automated Cell-Free Multiprotein Synthesis Facilitates the Identification of a Secretory, Oligopeptide Elicitor-Like, Immunoreactive Protein of the Oomycete Pythium insidiosum.

Antibacterial Discovery: 21st Century Challenges.

It has been practically 50 years for the reason that golden age of antibiotic discovery (1945-1975) ended; but, we nonetheless battle to establish novel drug targets and to ship new chemical lessons of antibiotics to exchange these rendered out of date by drug resistance.  Clearly, discovering new lessons of antibiotics is basically onerous, so repeating the previous methods, whereas anticipating completely different outcomes, appears to boarder on madness. The important thing questions handled on this assessment embody:

Phospho-ZAP-70 (Tyr493) Blocking Peptide
AF3313-BP 1mg
EUR 195
ZAP-70 Antibody
AF6312 200ul
EUR 304
Description: ZAP-70 Antibody detects endogenous levels of total ZAP-70.
ZAP-70 Antibody
AF6313 200ul
EUR 304
Description: ZAP-70 Antibody detects endogenous levels of total ZAP-70.
ZAP- 70 Antibody
ABF6312 100 ug
EUR 438
ZAP- 70 Antibody
ABF6313 100 ug
EUR 438
ZAP- 70 Antibody
ABD13372 100 ug
EUR 438
Zap 70 antibody
20R-2009 50 ug
EUR 281
Description: Rabbit polyclonal Zap 70 antibody
Zap 70 antibody
20R-2010 50 ug
EUR 281
Description: Rabbit polyclonal Zap 70 antibody
Zap 70 antibody
20R-2340 50 ug
EUR 281
Description: Rabbit polyclonal Zap 70 antibody
Zap 70 antibody
20R-2341 50 ug
EUR 281
Description: Rabbit polyclonal Zap 70 antibody
Tyrosine-Protein Kinase ZAP-70 (ZAP-70) Antibody
abx139944-01mg 0.1 mg
EUR 384
  • Shipped within 5-12 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP-70) Antibody
abx140043-01mg 0.1 mg
EUR 425
  • Shipped within 5-12 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP-70) Antibody
abx012369-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.
Monoclonal ZAP-70 Antibody
AMM03191G 0.1ml
EUR 484
Description: A Monoclonal antibody against Human ZAP-70. The antibodies are raised in Mouse. This antibody is applicable in WB, IP
ZAP-70 Polyclonal Antibody
ES7532-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA, WB, ELISA
ZAP-70 Polyclonal Antibody
ES7532-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse/Rat. This antibody is tested and validated for IHC, IF, WB, ELISA, WB, ELISA
ZAP-70 Polyclonal Antibody
ES7533-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse. This antibody is tested and validated for IHC, WB, ELISA
ZAP-70 Polyclonal Antibody
ES7533-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse. This antibody is tested and validated for IHC, WB, ELISA
ZAP-70 Polyclonal Antibody
ES7534-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
ZAP-70 Polyclonal Antibody
ES7534-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
ZAP-70 Polyclonal Antibody
ES7535-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
ZAP-70 Polyclonal Antibody
ES7535-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
ZAP-70 Polyclonal Antibody
ES8738-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human. This antibody is tested and validated for IHC, WB, ELISA
ZAP-70 Polyclonal Antibody
ES8738-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 from Human. This antibody is tested and validated for IHC, WB, ELISA
ZAP-70 (pY493) Antibody
20-abx119340
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.
ZAP-70 Polyclonal Antibody
ABP60950-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from part region of human ZAP-70 protein at amino acid sequence of 391-440
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human. This ZAP-70 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human ZAP-70 protein at amino acid sequence of 391-440
ZAP-70 Polyclonal Antibody
ABP60950-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from part region of human ZAP-70 protein at amino acid sequence of 391-440
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human. This ZAP-70 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human ZAP-70 protein at amino acid sequence of 391-440
ZAP-70 Polyclonal Antibody
ABP60950-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from part region of human ZAP-70 protein at amino acid sequence of 391-440
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human. This ZAP-70 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human ZAP-70 protein at amino acid sequence of 391-440
ZAP-70 Polyclonal Antibody
ABP56533-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y493
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse, Rat. This ZAP-70 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y493
ZAP-70 Polyclonal Antibody
ABP56533-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y493
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse, Rat. This ZAP-70 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y493
ZAP-70 Polyclonal Antibody
ABP56533-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y493
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse, Rat. This ZAP-70 antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y493
ZAP-70 Polyclonal Antibody
ABP56534-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y315
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse. This ZAP-70 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y315
ZAP-70 Polyclonal Antibody
ABP56534-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y315
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse. This ZAP-70 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y315
ZAP-70 Polyclonal Antibody
ABP56534-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y315
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse. This ZAP-70 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y315
ZAP-70 Polyclonal Antibody
ABP56535-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y319
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse, Rat. This ZAP-70 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y319
ZAP-70 Polyclonal Antibody
ABP56535-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y319
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse, Rat. This ZAP-70 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y319
ZAP-70 Polyclonal Antibody
ABP56535-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y319
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse, Rat. This ZAP-70 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y319
ZAP-70 Polyclonal Antibody
ABP56536-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y292
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse. This ZAP-70 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y292
ZAP-70 Polyclonal Antibody
ABP56536-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y292
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse. This ZAP-70 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y292
ZAP-70 Polyclonal Antibody
ABP56536-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y292
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 from Human, Mouse. This ZAP-70 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the non-phosphorylation site of Y292
ZAP-70 (pY319) Antibody
abx012370-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.
ZAP-70 (pY493) Antibody
abx012371-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.
ZAP-70 Polyclonal Antibody
46858-100ul 100ul
EUR 252
ZAP-70 Polyclonal Antibody
46858-50ul 50ul
EUR 187
Anti-ZAP-70 antibody
STJ98456 100 µl
EUR 234
Description: Mouse monoclonal to ZAP-70.
Anti-ZAP-70 antibody
STJ98801 200 µl
EUR 197
Description: Rabbit polyclonal to ZAP-70.
Anti-ZAP-70 antibody
STJ99214 200 µl
EUR 197
Description: Mouse monoclonal to ZAP-70.
Anti-ZAP-70 antibody
STJ96294 200 µl
EUR 197
Description: Rabbit polyclonal to ZAP-70.
Anti-ZAP-70 antibody
STJ96295 200 µl
EUR 197
Description: Rabbit polyclonal to ZAP-70.
Anti-ZAP-70 antibody
STJ96296 200 µl
EUR 197
Description: Rabbit polyclonal to ZAP-70.
Anti-ZAP-70 antibody
STJ96297 200 µl
EUR 197
Description: Rabbit polyclonal to ZAP-70.
Anti-ZAP-70 antibody
STJ16101150 100 µg
EUR 524
Anti-ZAP-70 antibody
STJ180268 0.1 ml
EUR 212
Phospho-ZAP-70 (Tyr319) Antibody
AF3312 200ul
EUR 304
Description: Phospho-ZAP-70 (Tyr319) Antibody detects endogenous levels of ZAP-70 only when phosphorylated at Tyrosine 319.
Phospho-ZAP-70 (Tyr493) Antibody
AF3313 200ul
EUR 304
Description: Phospho-ZAP-70 (Tyr493) Antibody detects endogenous levels of ZAP-70 only when phosphorylated at Tyrosine 493.
Phospho- ZAP- 70 (Tyr319) Antibody
ABF3312 100 ug
EUR 438
Phospho- ZAP- 70 (Tyr493) Antibody
ABF3313 100 ug
EUR 438
Zap-70 (Phospho-Tyr319) Antibody
11159-100ul 100ul
EUR 252
Zap-70 (Phospho-Tyr319) Antibody
11159-50ul 50ul
EUR 187
Zap-70 (Phospho-Tyr493) Antibody
11160-100ul 100ul
EUR 252
Zap-70 (Phospho-Tyr493) Antibody
11160-50ul 50ul
EUR 187
Zap-70 (Ab-319) Antibody
21173-100ul 100ul
EUR 252
Zap-70 (Ab-319) Antibody
21173-50ul 50ul
EUR 187
Zap-70 (Ab-493) Antibody
21174-100ul 100ul
EUR 252
Zap-70 (Ab-493) Antibody
21174-50ul 50ul
EUR 187
Anti-Human Zap-70 antibody
STJ16100440 1 mL
EUR 964
Zap-70 (Ab-319) Conjugated Antibody
C21173 100ul
EUR 397
ZAP-70 (phospho Tyr319) Polyclonal Antibody
ES7528-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr319) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
ZAP-70 (phospho Tyr319) Polyclonal Antibody
ES7528-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr319) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
ZAP-70 (phospho Tyr493) Polyclonal Antibody
ES7529-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr493) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
ZAP-70 (phospho Tyr493) Polyclonal Antibody
ES7529-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr493) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
ZAP-70 (phospho Tyr292) Polyclonal Antibody
ES7530-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr292) from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA
ZAP-70 (phospho Tyr292) Polyclonal Antibody
ES7530-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr292) from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA
ZAP-70 (phospho Tyr315) Polyclonal Antibody
ES7531-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr315) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
ZAP-70 (phospho Tyr315) Polyclonal Antibody
ES7531-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against ZAP-70 (phospho Tyr315) from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA
ZAP-70 (phospho Tyr319) Polyclonal Antibody
ABP56529-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y319
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr319) from Human, Mouse, Rat. This ZAP-70 phospho Tyr319) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y319
ZAP-70 (phospho Tyr319) Polyclonal Antibody
ABP56529-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y319
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr319) from Human, Mouse, Rat. This ZAP-70 phospho Tyr319) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y319
ZAP-70 (phospho Tyr319) Polyclonal Antibody
ABP56529-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y319
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr319) from Human, Mouse, Rat. This ZAP-70 phospho Tyr319) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y319
ZAP-70 (phospho Tyr493) Polyclonal Antibody
ABP56530-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y493
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr493) from Human, Mouse, Rat. This ZAP-70 phospho Tyr493) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y493
ZAP-70 (phospho Tyr493) Polyclonal Antibody
ABP56530-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y493
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr493) from Human, Mouse, Rat. This ZAP-70 phospho Tyr493) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y493
ZAP-70 (phospho Tyr493) Polyclonal Antibody
ABP56530-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y493
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr493) from Human, Mouse, Rat. This ZAP-70 phospho Tyr493) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y493
ZAP-70 (phospho Tyr292) Polyclonal Antibody
ABP56531-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y292
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr292) from Human, Mouse. This ZAP-70 phospho Tyr292) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y292
ZAP-70 (phospho Tyr292) Polyclonal Antibody
ABP56531-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y292
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr292) from Human, Mouse. This ZAP-70 phospho Tyr292) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y292
ZAP-70 (phospho Tyr292) Polyclonal Antibody
ABP56531-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y292
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr292) from Human, Mouse. This ZAP-70 phospho Tyr292) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y292
ZAP-70 (phospho Tyr315) Polyclonal Antibody
ABP56532-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y315
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr315) from Human, Mouse. This ZAP-70 phospho Tyr315) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y315
ZAP-70 (phospho Tyr315) Polyclonal Antibody
ABP56532-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y315
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr315) from Human, Mouse. This ZAP-70 phospho Tyr315) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y315
ZAP-70 (phospho Tyr315) Polyclonal Antibody
ABP56532-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y315
  • Applications tips:
Description: A polyclonal antibody for detection of ZAP-70 phospho Tyr315) from Human, Mouse. This ZAP-70 phospho Tyr315) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human ZAP-70 around the phosphorylation site of Y315
Anti-Zap-70 (Ab-319) Antibody
A00754 100ul
EUR 397
Description: Rabbit Polyclonal Zap-70 (Ab-319) Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.
Anti-Zap-70 Antibody (Monoclonal, 11H40)
M00754 100ug/vial
EUR 397
Description: Mouse Monoclonal Zap-70 Antibody (Monoclonal, 11H40). Validated in IHC, WB and tested in Human, Rat.
Anti-Phospho-ZAP-70 (Y319) antibody
STJ90436 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-ZAP-70 (Y319).
Anti-Phospho-ZAP-70 (Y493) antibody
STJ90437 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-ZAP-70 (Y493).
Anti-Phospho-ZAP-70 (Y292) antibody
STJ90483 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-ZAP-70 (Y292).
Anti-Phospho-ZAP-70 (Y315) antibody
STJ90842 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-ZAP-70 (Y315).
Zap-70 (Phospho-Tyr319) Polyclonal Conjugated Antibody
C11159 100ul
EUR 397
Zap-70 (Phospho-Tyr493) Polyclonal Conjugated Antibody
C11160 100ul
EUR 397
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
20-abx127086
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
20-abx001807
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
20-abx141278
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
20-abx141279
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx036840-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx036841-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx025459-100ul 100 ul
EUR 523
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx027828-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx027828-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx027829-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx027829-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx016023-100ul 100 ul
EUR 411
  • Shipped within 5-10 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx431723-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx239582-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx239583-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody
abx239584-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.
ZAP-70 (pY315) Cell ELISA Kit
abx596012-296tests 2 × 96 tests
EUR 707
  • Shipped within 1-2 weeks.
Tyrosine-Protein Kinase ZAP-70 (ZAP70) Antibody (FITC)
abx411157-25ug 25 ug
EUR 356
  • Shipped within 1 week.
Custom Peptide Synthesis (>70% antigen grade; mass spec, hplc (mg-kg size, price based upon peptide size: 2-100 aa)
PEP-70 1 Ask for price
Mouse Anti-Human ZAP-70 monoclonal antibody, clone JID181
CABT-L2812-100uL500uL 100 uL, 500 uL
EUR 502
Rabbit Anti-Human ZAP-70 Polyclonal Antibody, Phospho-Tyr319
CPB-713RH 100 ul
EUR 559
Rabbit Anti-Human ZAP-70 Polyclonal Antibody, Phospho-Tyr493
CPB-714RH 100 ul
EUR 559
DiagNano Fluorophore Labeled Gold Nanoparticles, 70 nm
GFL-70 1 mL
EUR 1053
DiagNano Gold Nanoparticle Passive Conjugation Kit, 70 nm
GPK-70 1 kit
EUR 715
Mouse Tyrosine-protein kinase ZAP-70 (ZAP70) ELISA Kit
abx520704-96tests 96 tests
EUR 739
  • Shipped within 5-12 working days.
ELISA kit for Human Tyrosine-protein kinase ZAP-70
EK4781 96 tests
EUR 670
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Tyrosine-protein kinase ZAP-70 in samples from serum, plasma, tissue homogenates and other biological fluids.
ZAP-70 (Phospho-Tyr292) Colorimetric Cell-Based ELISA Kit
EKC2136 100ul
EUR 572
ZAP-70 (Phospho-Tyr315) Colorimetric Cell-Based ELISA Kit
EKC2137 100ul
EUR 572
ZAP-70 (Phospho-Tyr493) Colorimetric Cell-Based ELISA Kit
EKC2540 100ul
EUR 572
Mouse Zap70/ Tyrosine-protein kinase ZAP-70 ELISA Kit
E1605Mo 1 Kit
EUR 632
Human ZAP70/ Tyrosine-protein kinase ZAP-70 ELISA Kit
E2710Hu 1 Kit
EUR 605
Human ZAP70(Tyrosine-protein kinase ZAP-70) ELISA Kit
EH2375 96T
EUR 567.6
  • Detection range: 0.312-20 ng/ml
  • Uniprot ID: P43403
  • Alias: ZAP70/mrtle/mur/SRK/STD/TZK/70 kDa zeta-associated protein/EC 2.7.10/EC 2.7.10.2/SRKFLJ17679/STDFLJ17670/Syk-related tyrosine kinase/tyrosine-protein kinase ZAP-70/TZK/ZAP-70/zeta-chain(TCR) a
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml
Mouse Tyrosine- protein kinase ZAP- 70, Zap70 ELISA KIT
ELI-07597m 96 Tests
EUR 865
Human Tyrosine- protein kinase ZAP- 70, ZAP70 ELISA KIT
ELI-07598h 96 Tests
EUR 824
Human Tyrosine-protein kinase ZAP-70 (ZAP70) ELISA Kit
abx251734-96tests 96 tests
EUR 739
  • Shipped within 5-12 working days.
Rabbit Anti-Human Zap-70/Syk (Phospho Y319/Y352) polyclonal antibody
CABT-BL131 100 ul
EUR 585
ZAP-70 (Chronic Lymphocytic Leukemia Marker); Clone ZAP70/528 (Concentrate)
RA0453-C.1 0.1 ml
EUR 125
ZAP-70 (Chronic Lymphocytic Leukemia Marker); Clone ZAP70/528 (Concentrate)
RA0453-C.5 0.5 ml
EUR 300
ZAP-70 (Chronic Lymphocytic Leukemia Marker); Clone ZAP70/528 (Concentrate)
RA0453-C1 1 ml
EUR 500
Phospho-ZAP-70 (Tyr493) Colorimetric Cell-Based ELISA Kit (OKAG02084)
OKAG02084 2 x 96 Wells
EUR 740
Description: Description of target: ;Species reactivity: Human: Y493, Mouse: Y492, Rat: Y488;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: Phospho
Detection Method: Colorimetric 450 nm;Sensitivity:
Phospho-ZAP-70 (Tyr292) Colorimetric Cell-Based ELISA Kit (OKAG01664)
OKAG01664 2 x 96 Wells
EUR 740
Description: Description of target: ;Species reactivity: Human: Y292, Mouse: Y290,;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: Phospho
Detection Method: Colorimetric 450 nm;Sensitivity:
Phospho-ZAP-70 (Tyr315) Colorimetric Cell-Based ELISA Kit (OKAG01665)
OKAG01665 2 x 96 Wells
EUR 740
Description: Description of target: ;Species reactivity: Human: Y315, Mouse: Y314;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: Phospho
Detection Method: Colorimetric 450 nm;Sensitivity:
C3a (70 77) Peptide
20-abx265956
  • EUR 384.00
  • EUR 592.00
  • EUR 300.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
IL2 (60-70) Peptide
20-abx266260
  • EUR 467.00
  • EUR 759.00
  • EUR 342.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
Erythropoietin Mimetic Peptide Sequence 20
H-4344.0001 1.0mg
EUR 454
Description: Sum Formula: C72H99N17O17S2; CAS# [203397-62-0] net
Erythropoietin Mimetic Peptide Sequence 20
H-4344.0005 5.0mg
EUR 1723
Description: Sum Formula: C72H99N17O17S2; CAS# [203397-62-0] net
Substance P reversed sequence Peptide
20-abx266314
  • EUR 495.00
  • EUR 815.00
  • EUR 356.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
Zap70 ELISA Kit| Mouse Tyrosine-protein kinase ZAP-70 ELISA Kit
EF016523 96 Tests
EUR 689

Is the variety of novel chemical lessons of antibacterials restricted by the variety of broad spectrum drug targets; and (3) If true, then ought to we focus efforts on subgroups of pathogens like Gram unfavourable or optimistic micro organism solely, anaerobic micro organism or different group the place the vary of widespread important genes is probably going higher?. This assessment additionally supplies some examples of present drug targets that seem to flee the specter of mutation based mostly drug resistance, and supplies examples of some intermediate spectrum methods in addition to fashionable molecular and genomic approaches possible to enhance the chances of delivering 21st century medicines to fight multidrug resistant pathogens.